Site web : http://www-lbme.biotoul.fr/uk/equipes/grpcuvier/

Unité d'appartenance : Responsable d'unité : M. Ferrer

Code unité : UMR5099              Institut :CNRS  Ville : Toulouse       Délégation régionale : DRH-Toulouse  

Nom de l'équipe : Cell Cycle Chromosome Dynamics

Thématique de l'équipe (< 10 lignes) :

Our research group has identified the Drosophila BEAF chromatin insulators (>2,000 elements) that we are currently characterizing. BEAF/CTCF insulators belong to Class I insulators that may function through common mechanisms and partners that we have biochemically identified. In particular, we test whether such factors function to regulate the expression of essential oncogenes through: a) the local organization of chromatin, nucleosome-positioning and/or the propagation of repressive chromatin/histone marks b) through long-range DNA contacts that may regulate transcription, for example by limiting enhancer-promoter interactions. 

Techniques utilisées :

Chromosome Capture Conformation (3C)

Statistical and Bioinformatic approaches

ChIP, DGE, RNA-seq, qPCR

RNAi, Cell Biology, Cytometry, Microscopy

Cytometric microscopy (ScanR)

Mots clés (thématiques et/ou techniques; <10) :

Chromatin insulators, Nucleosome positioning, Nuclear bodies, Long-range DNA interactions