Mail: leh@lbpa.ens-cachan.fr

Site web : http://www.lbpa.ens-cachan.fr/version-francaise/equipes/dynamique-des-complexes-macromoleculaires/

Unité d'appartenance : UMR 8113 (LBPA)

Responsable d'unité : Jean-Françoise Mouscadet (Malcolm Buckle)

Code unité : UMR8113           Institut : CNRS                Section principale : 21

Ville : Cachan             Délégation régionale : Ile de France 

Nom de l'équipe : Dynamique des complexes macromoléculaires

Thématique de l'équipe (< 10 lignes) : Chromatin Organisation: a Regulatory Dynamic Analysis. Chromatin structure is both dynamic and highly organised. The major goal of this project is to investigate the nature of structural transitions both at the local level of promoter structure and at the global level of folding and unfolding of the chromatin fibre. Design and implementation of novel biosensors: a) The design, elaboration, characterization and validation of a polymer-based, label-free optofluidic sensor for chemical and biological applications, focused on real-time investigation of the kinetics of specific macromolecular interactions (protein-protein and protein-nucleic acid). b) The design and implementation of a MOlecular Controlled Semiconductor Resistor (Mocser)

Techniques utilisées :

UV laser photofootprinting; AFM; SPR et SPRi; Microresonator optofluidic biosensors; Real time DNA footprinting; robotics (spotters and micro manipulation), clean room surface chemistry techniques (UVO; RIE nano deposition...); Capillary DNA sequencing; Mass sepctrometry (SELDI and ESItof).

Mots clés (thématiques et/ou techniques; <10) :

Chromatin; Regulation of Gene expression; Biosensors; Macromolecular complexes; dynamics of nucleoprotein complex formation. Laser photofootrpinting; Surface Plasmon Resonance.