GHAVI-HELM Yad

Fonction : CRCN CNRS

Tél : 04.26.73.13.50

Mail : yad.ghavi-helm@ens-lyon.fr

 

Unité d'appartenance : Institut de Génomique Fonctionnelle de Lyon (IGFL)

Responsable d'unité : Florence Ruggiero

Code unité : UMR5242     Institut : CNRS, ENS de Lyon , UBCL, INRAE     Section principale : 22

Ville : Lyon     Délégation régionale : DR7

 

Nom de l'équipe : Developmental Epigenomics

Responsable : Yad GHAVI-HELM

Site web : https://www.ghavihelmlab.com/      http://igfl.ens-lyon.fr/equipes/y.ghavi-helm-developmental-epigenomics

 

Thématique de l'équipe (< 10 lignes) : In eukaryotes, the complex regulation of temporal- and tissue-specific gene expression is controlled by the binding of transcription factors to enhancers, which in turn interact with the promoter of their target gene(s) via the formation of a chromatin loop. Despite their importance, the properties governing enhancer function and enhancer-promoter loops in the context of the three-dimensional organization of the genome are still poorly understood. My group applies genetics, genomics, and computational  biology methods including novel single-cell techniques, using Drosophila embryogenesis as a model system to gain insights into the functionality of enhancer-promoter interactions in a developing embryo, an important step towards understanding the link between chromatin organization and transcription regulation.

Techniques utilisées : ChIP-seq, 4C-seq et Hi-C, RNA-seq et dérivés pour identification des transcrits naissants, single-cell RNA-seq, ATAC-seq, élevage de Drosophile et microinjection, FACS, hybridation in situ

Mots clés (thématiques et/ou techniques) : Chromatine, enhancer, organisation de la chromatine, interactions enhancer-promoteur, Drosophile, embryogenèse