Fonction : CR1/CNRS

Tél : 03 88 65 32 45   Fax : 03 88 65 32 01

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Unité d'appartenance :

Responsable d'unité : Brigitte Kieffer

Code unité : CNRS UMR 7104 - Inserm U 964    Institut : IGBMC Section principale : 22 (+20,21,24,25)

Ville : Illkirch    Délégation régionale : Alsace

Nom de l'équipe : Biologie cellulaire de l'intégrité du génome

Thématique de l'équipe (< 10 lignes) : Each cell in the human body receives thousands of DNA lesions per day. DNA lesions can interfere with genome replication and transcription, and if they are not repaired or are repaired incorrectly, they lead to mutations that may threaten cell viability. The most deleterious DNA breaks are the Double Strand Breaks (DSBs) because unfaithful repair can lead to the formation of cancerous chromosomal translocations. It is poorly understood why translocations between chromosomes recur at specific break points in the genome and even less is known about how ends from different DSBs meet in the cell nucleus. It was recently shown that broken chromosome ends are positionally stable and unable to roam the cell nucleus and that unrepaired DSBs preferentially undergo translocations with neighboring chromosomes. In our group we are using a unique cell system to induce DSB at a specific chromosomal location and to follow the fate of damaged DNA in living cells in real time. Our goal is to investigate the dynamics of DSBs in relation to the surrounding chromatin structure and nuclear architecture and to test how this is related to their repair and their involvement in the formation of chromosomal translocations.

Techniques utilisées :

Immunofluorescence, FISH, live Imaging, Chromatin IP.

Mots clés (thématiques et/ou techniques; <10) :

DNA repair, chromatin, nuclear architecture, chromosomes, FISH, imaging